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The blood sample bottles were processed in parallel by conventional methods.
Briefly, sections were processed in parallel using identical antibody concentrations.
Control slices were processed in parallel without addition of UCB.
Multiple (at least two) chips were processed in parallel.
Negative DNA extractions were processed in parallel to rock and water sample extractions.
Within each experiment samples were processed in parallel using the same reagents.
Negative controls were processed in parallel by replacing the primary antibody by buffer.
Nnd1-dse and the wild type embryos were processed in parallel and in an identical manner.
Mock specimens were processed in parallel with patient specimens to monitor for reagent contamination.
All sections from each experimental animal and group to be compared were processed in parallel.
Control non-injected or saline-injected and WT mouse tissues were processed in parallel.
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