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Blood parameters were proceeded for hematological analysis using a Nihon Kohden automated hematology analyzer (model MEK-6318K, Japan), including leucocytes (WBC) (103/μl) and their differential relative% (neutrophils, lymphocyte, basophils, and monocytes) in peripheral blood (PB).
Embryos were proceeded for fluorescent double in situ hybridization with protocols previously reported [32], [86].
To favor the temperature exchange between the dry bath and the samples, 1.2 ml aliquots were prepared and triplicates of aliquots were proceeded for each condition.
Therefore, all data were proceeded for phylogenetic analyses without modification.
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48 hours after transfection cells were proceed for RNA extraction using Trizol (Invitrogen).
Finally, 19 hits with good pharmacokinetic parameter and negligible toxicity was proceeded for synthesis.
The reaction was proceeded for 2 h at 130 °C under inert gas.
After the reaction had been proceeded for 1 h, the composition of the FePt particles changed obviously.
Then, the IBE was proceeded for an extra 5 min to transfer the nanopattern of BL mask to the PZT film (Fig. 1a(ii)).
Such cycles was proceeded for 8 levels to ensure reaching the depth of bulk Al.
The solutions were thoroughly deoxygenated under an argon atmosphere and the reaction was proceeded for 5 h at 50 °C.
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