Citrus species contributed to a global production of 131 million tons of fruit harvested over 8.7 million hectares in 2011 (FAOSTAT, 2013), and are primarily utilised for juice making and fresh fruit consumption.
In the past, several successful methods were designed with varying degrees of correspondence with biological vision studies, ranging from purely functional inspiration to methods that utilise models that were primarily developed for explaining biological observations.
Most previous reports on the expression of c- myc mRNA have utilised Northern blot, dot blot or PCR-based approaches, while just a few involved in situ hybridisation, which were primarily performed on frozen tissue sections (Liao and Dickson, 2000).
The customers were primarily fisherman.
This work is primarily focused in utilising a technique to assess low cycle fatigue life of steam turbine blade.
The studies described below have primarily utilised longitudinal designs in which subjects were followed across a training program.
Cancer epigenome studies to date have primarily utilised cancer cell lines or clinical samples, where it is difficult to identify the initial epigenetic lesions from those that occur over time.
This research primarily utilised data from focus group discussions for analysis, two of the FGDs were conducted in Hindi, and an independent researcher did the translation and transcription, with a separate researcher performing analysis.
During that study method validation was conducted in accordance with available internationally recognised bioanalytical guidelines established by the pharmaceutical industry, but primarily utilised in drug and safety monitoring (Shah et al, 2000; Miller et al, 2001).
This is primarily due to the small number of studies, and a failure to utilise robust study designs, sample sizes and appropriate statistical analyses.
