Exact(18)
The CYC2 gene knock-out (KO) strains (ΔCYC2) of two mating types (CU428 and B2086 II) were previously constructed and well-tested (Hayashi and Mochizuki, 2015).
The N-terminal fragments of SspDnaE intein (SspDnaE-IntN) of various lengths were previously constructed [24].
GFP-EHD1 [20], GFP-EHD3 [24], YFP-EHD2 and YFP-EHD4 were previously constructed in the lab.
Magel2-null mice were previously constructed by gene-targeted replacement of the open reading frame of Magel2 with a lacZ reporter cassette [13], and are maintained on a C57BL/6 background.
Thus, we tested if the ALP compounds also block the stress-induced Akt phosphorylation of the CHME5 sublines that were previously constructed by stably transfecting with pCDNA3.1-hygro [ Tat] or pTat101 [ Tat] expressing the 101 amino acid M-tropic YU-2 Tat protein [19].
To express MsrB1 in mammalian cells, the following constructs were generated: wild-type and cysteine (Cys) mutant cDNA of mouse MsrB1 (NM_013759) corresponding to Form 1 3'-UTR were amplified from pET28a MsrB1-Sec and pET28a MsrB1-Cys as templates that were previously constructed in the laboratory.
Similar(42)
Such structures have been previously constructed at the Jet Propulsion Laboratory focusing on large scale booms.
The ejector, with movable primary nozzle, was mounted on the system that was previously constructed for low pressure refrigerants.
A total cerebrovascular disease (CeVD) burden scale was previously constructed and an inverse association of CeVD burden and cognition was found.
pGEM-3Z-5'UTR was previously constructed [18].
The nrdAB mutant strain had been previously constructed in our lab.
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