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Construction and validation of vector-based siRNA probes to suppress the endogenous EB1 or KIF3A levels in rat hippocampal neurons were previous described [19].
Similar reductions in ERG amplitude were previous described by Wagh et al. (2006) using RNAi against brp driven by GMR-GAL4, another eye-specific driver.
The spectral properties of CPN were previous described and the absorption maximum is centered at 438 nm and emission maximum is at 483 nm [ 23].
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Ad5-CRAD and Ad5-PGK-GFP have been previous described [6], [9].
The ebp1 expression construct has been previous described (Xia et al, 2001a).
The method of amniotic membrane preparation has been previous described by Kim and Tseng [ 18].
Immortalized human keratinocyte cell line HaCaT derived from human adult trunk skin was previous described [ 27, 28].
The PCR primers used for amplifying β-globin, XMRV and MCV have been previous described (Andres et al, 2010; Erlwein et al, 2010).
Procedures for quantitative mass spectrometry sample preparation and data analysis were as previous described [ 42, 43].
Ex vivo degradation assays were performed as previous described (Bard et al., 2000).
Podocyte were cultured as previous described [6].
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