Afterwards, proteins were precipitated with 100 μl of protein precipitation solution (Qiagen).
DNA/proteins were precipitated with ethanol, air-dried and dissolved in 100 ul of TE.
Whole saliva and iSG-derived saliva proteins were precipitated with acetone.
Supernatants were precipitated with 5 volumes and ethanol and air-dried before use.
Whole cell lysates were precipitated with WGA-agarose beads (A), or anti-FoxO1 (B) or anti-O-GlcNAc (C) antibody.
At the last washing step, the microspheres were precipitated with 10 mM Tris-HCl, 150 mM NaCl, at pH 7.4.
The lysates were precipitated with anti-GFP antibody.
The proteins were precipitated with cooled acetone and lyophilized.
After 5-min mixing, the QDs in the suspension were precipitated with the addition of acetone.
Thereafter, the crude BoNT/A were precipitated with 60% ammonium sulfate at 4 °C overnight.
After incubation, proteins were precipitated with acetonitrile/ethanol (1 1) and TFA (0.1%) and then centrifuged.
