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Cells were plated on coated poly-D-lysine coverslips and transduced as described above.
The transformants were plated on SC-Ura media with 1% (w/v) maltose (SCM-Ura).
For live-cell imaging, cells were plated on a confocal dish (MatTek corporation, USA).
4×105 cells were plated on top of it, in 0.3% agar.
Cells were plated on PDL-coated cover glass, transfected and starved as described earlier.
After 40 passages, cells were plated on solid YNB medium.
U87 cells were plated on microscopic glass slides.
These were plated on LB with ampicillin.
Transformed cells were plated on minimal media.
MCF7 cells were plated on glass coverslips.
Serial dilutions from each well were plated on BCYET plates.
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