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All NOESY peaks were picked using the peak-picking mode in Sparky (http://www.cgl.ucsf.edu/home/sparky) for individual strips with noise and artefact peaks removed manually.

Colonies were picked using sterile cloning rings and expanded to form sub lines.

Protein spots representing greater than twofold alteration in expression were picked using an Ettan Spot Picker (GE Healthcare Biosciences).

Operational Taxonomic Unit (OTUs) were picked using de novo OTU picking protocol with a 97% similarity threshold and singletons and doubletons were discarded in the QIIME software.

632 sub-volumes with a box size of 84 pixels were picked using IMOD (Kremer et al., 1996) and extracted using RELION1.4 (Bharat et al., 2015).

The resulting transmitted waveforms were picked using a customized Graphic User Interface (GUI) and then corrected for zero-time and column shell delays.

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After incubation under 30 °C for 4 days, each colony was picked using inoculating loop and then transferred to another plate by streaking for further purification and growth.

The best agent is picked using a set of rules from a rule base, which is currently maintained manually by an expert.

Primers flanking the SSRs are picked using Primer3 (40).

Briefly, each protein spot on SYPRO Ruby stained gels was picked using a spot picker (Amersham Biosciences).

Once an MTP was picked using clones with paired BES, clones with unpaired BES were used only where we were unable to place clones with paired BESs.

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