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Intravital TPLSM recordings were performed upon first arrival of Tβ-syn or TMBP cells in the leptomeningeal brain vessels.
Statistical analyses were performed upon the time-series data from each functional run using a General Linear Model in FSL.
Alternatively, invasion assays were performed upon co-inoculation of wild-type (RH-2YFP) used as an internal control and the strain to be tested, as described previously20.
Full-scale damping measurements were performed upon three classes of civil engineering structures including free-standing lattice towers, reinforced concrete buildings and pedestrian footbridges.
For endogenous TFEB staining, cells were permeabilized with 0.1% Triton X-100 for 5 min, followed by blocking with 3% bovine serum albumin in PBS + 0.02% saponin for 1 h at RT. Immunostainings were performed upon dilution of primary antibodies in blocking solution and overnight incubation at 4 °C, followed by three washes and secondary antibody incubation in blocking solution for 1 h at RT.
In order to shed light upon the seismic behaviour (stiffness, strength, ductility, energy-dissipation) of such structures, a number of tests were performed upon two dimensional (3.0 m by 3.0 m) cast in situ sandwich squat concrete walls (with and without openings).
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Both of these techniques can be performed upon standard, industrial knitting machines with high-performance yarns such as glass and carbon.
To assess the performance and robustness of the whole framework, sensitivity analyses are performed upon varying key constitutive, loading and numerical parameters.
In the Sims' world, everything is an object that yields a measurable benefit when some action is performed upon it.
"A young man was made to inhale the vapour while an operation was performed upon the tissues near the eye.
Finally a Pareto optimal analysis is performed upon the data acquired from previous two processes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com