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Previous difficulties in expressing PfEMP1 domains in E.coli [17], [24], [25], [26], [27], [28] were overcome using the methods developed by Higgins [29].
For reasons that remain unknown, these issues were overcome using a strategy of foster mothering with FVB/NJ females.
Initial concerns regarding nonselective uptake of Qdots into the reticuloendothelial system and solubility were overcome using pegylation chemistry (Akerman et al, 2002), which results in a hydrophilic surface.
Potential problems with detection of low molecular weight restriction fragments i.e. <50 bp were overcome using the modified DNA polymerase I large (Klenow) fragment end-labelling, but ideally the use of radiolabelled or fluorescently labelled degenerate PCR primers would obviate downstream labelling reactions and help solve such problems.
Similar(56)
Low levels of noise may be overcome using additional absorbing material, such as heavy drapery or sound-absorbent tiles in enclosed rooms.
This shortcoming is overcome using fuzzy or interval models.
These problems could be overcome using immobilized yeast.
However, such difficulty can be overcome using the following method.
This can easily be overcome using free file converters.
This problem has been overcome using silver colloid as drug carrier.
The drawback faced by ADF can be overcome using the DDF scheme.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com