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Histological analyses revealed that HFH appeared as clusters of columnar epithelial cells that were organizing into duct-like structures in gels prepared by using all 4 protocols: Bcl-2-HUVEC and rCI/hFN, Bcl-2-HUVEC and rCI/hFN/mLBM, Bcl-2+HGF-HUVEC and rCI/hFN, and Bcl-2+HGF-HUVEC and rCI/hFN/mLBM (Fig. 7A 7D, respectively, white arrows) by 2 weeks post-engraftment.
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