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Then screened variables were optimized by Box Behnken experiment design.
Parameters of MAE were optimized by response surface methodology.
The 3 selected factors were optimized by response surface methodology.
The preparation experiments were optimized by Taguchi designs.
After that, the crucial fermentation parameters affecting polygalacturonase activity were optimized by response surface methodology (RS M.
Those 3D structures were optimized by energy minimization with SYBYL- × 2.0.
Fluorescence microscopy images were optimized by adjusting the contrast and afterwards binarized by conversion to 8-bit images.
All images were optimized by using a fluorescence microscope or a laser scanning confocal microscope (Nikon, http://www.nikonusa.com/).
Both experimental setups used for OW and PA measurements were optimized by maximizing the optical alignment.
The methods were optimized by use of the results of molecular biological experiments.
The reaction conditions were optimized by determining the optimal annealing temperature and primer concentration.
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