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Notably, although sgRNAs corresponding to BRCA1-A complex genes were enriched in topotecan-resistant Atm−/− cells, sgRNAs targeting Brca1 or the genes for factors present in other BRCA1-containing complexes were not (Supplementary Data file 3).

In agreement with 32Hc activity requirement for DNA-PKcs kinase, we found that DNA-PKcs proficient cell lines M059K and F18 were radiosensitized by transfection with 32Hc while DNA-PKcs deficient cell lines M059J, KA4 and V3 were not (Supplementary Fig. S2B).

LOX, LOXL1, and LOXL2 mRNAs were detected in these cells but LOXL3 and LOXL4 transcripts were not (Supplementary Figure 1).

Although this common pathway was found, common genes within the pathway were not (Supplementary data, Table S2).

Ten genes (Anxa10, Clu, Gpr137b, Poll, Prnp, Sacs, Sgsh, Slc16a3, Tnfrsf19 and Tyro3) were selected using these criteria, whereas others were not (Supplementary Fig. S1).

Moreover, the protein level of PDH2 was increased upon OA treatment for 36 h, instead PDH1 and PHD3 were not (Supplementary Figure 2D).

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The opposite was seen with FAM46C, where loss was significant for the outcome, but mutation was not (Supplementary Figure 5).

And though education was always a core part of the Met's mission, this design by Kevin Roche John Dinkeloo and Associates firmly declares that education is not supplementary but central to the life of the Met.

Whereas translatability of the chimeric mRNA was affected by deleting XRN1 (Supplementary Fig. 2a), the 2a-Rluc protein turnover was not (Supplementary Fig. 2b).

Again, USP7 was capable of de-ubiquitinating H2Aub, but USP11 was not (Supplementary Figure S6C).

Unexpectedly, total STAT6 protein level was also decreased while the mRNA level of STAT6 was not (Supplementary information, Figure S6A).

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