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Animals were acclimated to laboratory conditions for 1 month prior to experimentation and fed a commercial diet ad libitum daily but were not fed for a minimum of 48 h prior to use in the experimentation.
The hatchery fish were not fed for 24 48 hours prior to sampling.
To avoid stomach bulge that could influence morphometrics, fish were not fed for 24 h prior to sampling.
The mice were not fed for 4 hours and were weighed before intraperitoneal glucose injections.
At the end of the trial, fish were not fed for 48 h prior to being anaesthetised and killed by overdose with metacaine sulphonate (MS222).
At the beginning of the experiment fish were separated randomly into two tanks, in one tank fish were fed ad libitum as before and in the other tank they were not fed for 4 weeks.
Similar(51)
"People were arrested, they were kettled, and they weren't fed for 11 hours.
But in the end it was as though these conversations had been conducted at the town pool, underwater, because John Cronin the Fish wasn't fed for nearly two weeks.
The cats weren't fed or cared for — they existed on their own.
Of even more concern to their parents (presumably), these same coddled children, when grown into adults, will have no idea how to persevere at a job if they are not fed constant affirmations for their work.
The dogs are not fed and have to scavenge for their food.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com