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ERα mRNA and protein levels were not affected upon treatment with the ERRα antagonist, but other ERRα target genes such as pS2, osteopontin, and aromatase mRNA levels decreased upon treatment with the ERRα-subtype specific ligand.
Also, ERα (ESR1) mRNA levels were not affected upon treatment with the ERRα antagonist, but other ERRα (ESRRA) target genes such as pS2 (TFF1), osteopontin (SPP1), and aromatase (CYP19A1) mRNA levels decreased.
The genetic homogeneity of the South Korean population, [29] the fact that the allele frequencies did not differ markedly between recruitment centers and the fact that the significance of the results were not affected upon controlling for genomic control inflation factor measured in other studies (1.061) [29] are reassuring.
Similarly, chromatin-bound SMARCA5 levels were not affected upon knockdown of Hdacs1,2.
Of note, p21 protein levels in p57−/− cells were not affected upon osmostress and were downregulated upon the reintroduction of p57 (Supplementary Figure S5B and C).
Fluorescent signals were not affected upon the HD mutation in AbdA, suggesting that AbdA-TFIIbeta interaction did not depend on AbdA DNA-binding.
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Preliminary tests verified that expression of PBGD was not affected upon treatment of NHEK and HaCaT keratinocytes.
The migration speed of the highly deformed cells inside the channels was three times higher than of cells on flat substrates but was not affected upon SPC treatment.
Consistent with the notion that blood may not comprise a severe stress for T. denticola, cell viability was not affected upon extended (up to 4 hours) of blood exposure, while all other conditions lead to a significant reduction during the same time period (data not shown).
However, localization of Tsr2-3xGFP was not affected upon eS26-depletion.
TRIAD1 binding to UBCH7 was not affected upon treatment with MLN4924.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com