Exact(5)
Immunoblots were next processed with appropriate secondary antibodies (either 1∶10000, HRP/goat anti-rabbit IgG, sc-2004, or 1∶10000 HRP/goat anti-mouse IgG, sc-2005, both from Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA, based on the primary antibody) for 1 hour at room temperature.
The firing patterns for each cluster as determined above were next processed by a global normalization and smoothing procedure designed to factor out slow fluctuations in the total neural activity (across all clusters) that could occur over the course of an experimental session or across sessions.
Reads not mapping to miRNAs were next processed as follows.
Both images were next processed by morphological and size filters to complete the shape of the nuclei and to discard formations less than 300 pixels in size; 300 was the lower threshold set for identifying a formation as nucleus and it was set experimentally.
The protocol for OP-puro labeling was adapted from Liu et al. Cells were incubated with 25 μM OP-puro for 45 min and CuAAC detection of OP-puro incorporated into nascent proteins was performed as previously described, using Alexa Fluor 594 azide (A10270, Life Technologies) (Liu J et al).. Cells were next processed for immunofluorescence microscopy as described above.
Similar(55)
The encoded sequence at the output of the BCH encoder is next processed through an inner LDGM code, that is, a linear code with low density generator matrix.
The COL1A1/PDGFB precursor is next processed to the oncoprotein PDGFB, which is thought to be at the origin of DFSP [ 11].
Most of the mtDNA genes are transcribed as almost genome-length polycistronic transcripts (see Box 2 ) that are next processed to produce individual mRNA and tRNA molecules.
The structural parameters within the ketene that govern the outcome of these chemodivergent reaction processes were next examined systematically.
Those areas (hydrology and geology) and their respective software that was mentioned are the next processes of scientific research to be studied and employed to be used for the purpose of forecasting.
The resulting protein retrieved from the sequence comparison is next analysed regarding process classification and hierarchy (either in biological terms or in technical terms), and a list of processes obtained.
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