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The tubes were next placed on a magnet holder (Invitrogen-Dynal) for 2 minutes before transferring the supernatant to a new tube and discarding AMPure XP particles from this first separation step.
Samples were next placed in serial dilutions until fixed in 100%% ethanol and embedded in paraffin.
Embryos were next placed at 4°C for different times before being returned to resume and complete development at room temperature.
Embryos were next placed in a Petri dish with the face anterior and the middle of the eyes even with the nasal pits.
To induce angiogenesis, 33 μl of either the culture medium to be tested or recombinant human basic Fibroblast Growth Factor (bFGF) (R&D Systems) (positive control) were next placed inside the rings on successive days for 3 days.
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The upper shear box with its roller bearings was next placed immediately above the lower sample, resting on the steel channels.
A white ball was next placed against the right-hand rail, directly opposite the lower bottle.
The tissue was next placed in a 1 1 solution of ethanol and toluene, then twice in toluene alone, each time for 2 hours at room temperature.
The samples are next placed in 10% neutral-buffered formalin (Shandon Formal-Fixx, Thermo Scientific, Waltham, MA) for a maximum of 90 min. This step enhances the red/pale contrast between viable AAR and AON (see Fig. 2, top).
They were convinced they were going to be the next place to be hit".
When the component to be worked is placed next to such a machine, the coil induces in it a corresponding field.
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CEO of Professional Science Editing for Scientists @ prosciediting.com