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The biggest problem, however, was that the antibodies were mouse proteins, which the human immune system attacked as invaders.
The T lymphocytes were mouse CD4 T cells from whole mouse splenocytes.
Primary antibodies used for detection of the Ab on Western blot and in enzyme-linked immunosorbent assay (ELISA) experiments were mouse anti-human kappa Ab or mouse anti-his Ab (Sigma-Aldrich, Bornem, Belgium).
The monoclonal antibodies used were mouse anti-human CD62L (MCA1076F) and CD11b (MCD11bF).
Primary antibodies used were: mouse monoclonal anti-α-tubulin and rabbit anti-troponin.
The primary antibodies used were mouse anti-α-COP polyclonal antibody and rabbit anti-GM130 polyclonal antibody (Abcam).
Antibodies used were mouse anti-CDC6 (sc-9964; Santa Cruz Biotechnology), goat anti-BMI1 (sc-8906; Santa Cruz Biotechnology).
In the WF analysis identified proteins were mouse SOD1, keratin and 2 of the heat shock protein 70 member proteins.
The primary antibodies used were mouse anti-BrdU (for detection of IdU, Becton Dickinson) and rat anti-CldU (Novus Biologicals).
The primary antibodies used were mouse monoclonal anti-α-tubulin and anti-β-actin (both from Sigma, 1∶200).
Primary antibodies used were mouse anti-BrdU (1∶1000, Sigma) and mouse anti-Tuj-1 (1∶500, Covance).
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