Sentence examples for were matured by from inspiring English sources
To test this notion, we generated autologous DC from the same donors from whom the CTL clones had been generated; the DC were matured by addition of a standard maturation cocktail consisting of IL-1β, IL-6, TNF-α, and PGE2 [35].
DCs were matured by overnight treatment with 1 µg/ml LPS (Sigma-Aldrich) and, where appropriate, pulsed with mBSA [50 µg/ml] for 4 hours prior to maturation.
The cells were then either taken as immature CD34DCs or were matured by culturing them in the growth medium supplemented with either 100 ng/mL LPS (Sigma) or DH5α bacteria (Stratagene) and allowing maturation to proceed for 48 hours.
The high expression levels of CD40 and CD86 in MGL2+DDCs indicated that these cells were matured by definition at the steady state as demonstrated previously for migratory CD11c+MHCIIhi DCs (Figure 4A) [35].
On day 8 in culture (day 4 post-transduction) moDCs were matured by stimulation with a "cytokine cocktail" made up from 5 ng/ml tumor necrosis factor α (TNFα), 5 ng/ml interleukin 1β (IL-1β), 150 ng/ml interleukin 6 (IL-6) (all R and D systems) and 1 µg/ml prostaglandin E2 (PGE2; Sigma).
Immature MUTZ3-LC were matured by adding 33% monocyte condition medium (MCM) and 2,400 IU/ml TNFα for 48 h.
Cybip is encoded by a single polymorphic gene and the native protein is matured by cleaving a signal peptide.
To activate immune system, DCs may be matured by many factors like bacterial CpG-DNA, Lipopolysaccharaide (LPS) and other microbial products.
Music historian Robert Stephens attributes the birth of soul music to "What'd I Say" when gospel and blues were successfully joined; the new genre of music was matured by later musicians such as James Brown and Aretha Franklin.
APC that are matured by a potent stimulus will not endocytose FITC-dextran particles efficiently, whereas a stimulus that does not mature the BMDCs will allow the cells to continuing sampling the microenvironment.
Human immature classical DCs that were generated from CD34+ hematopoietic progenitor cells or peripheral blood by cytokine stimulation could be matured by CD137 ligand agonists to inflammatory DCs [16] [18].
