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If no CAPS were previously designed from our SNP set, CAPS markers were manually designed with CAPS Designer (http://solgenomics.net/tools/caps_designer/caps_input.pl) and Primer3Plus (http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi/).
Although contact burns were frequently used models, most of them were manually designed.
Overlapping primers were manually designed at the exon-exon joining sites.
Primers were manually designed and their characteristics were estimated using FastPCR [29].
Primers used to analyse the expression of honeybee neuroligins and neurexin I were manually designed and then verified by PRIMER [103].
Sequences were manually designed and checked by means of the PrimerExpress™ program (Applied Biosystems) for the following parameters: secondary structure, melting temperature, GC content, primer-primer and primer-probe dimers.
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When the sequences are manually designed this knowledge comes from a human brain, aided by manual lookup of literature and databases.
The network topology was manually designed, and then modified in response to observed difficulties during evolution.
Firstly, a reasonable AI operating at Ku band was manually designed using a 130 nm technology.
These plans are manually designed by expert traffic controllers and are specific to a certain location and timetable.
Based on the experimental analysis about grip forces, a spring was manually designed and a linear motor was selected.
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