Cell cultures were maintained at pH 8.2 utilizing pH controlled mass flow valves supplementing CO2 as needed into the continuous air supply.
One-year old rooted seedlings of Yanshan-1 and Pinot Noir were maintained in a greenhouse, and were utilized for stress experiments.
Additionally, these lines can be maintained and utilized for phenotypic analysis.
Appropriate Healthcare Technology (AHT) refers to "methods, procedures, techniques and equipment that are scientifically valid, adapted to local needs and acceptable to those who use them and to those for whom they are used, and that can be maintained and utilized with resources the community or country can afford" [ 1].
Mice were maintained in specific pathogen-free conditions, and were utilized under protocols approved by the Duke University Institutional Animal Care and Use Committee.
The body temperature was maintained at 37° utilizing a heated air system (SA Instruments , Inc Stony Brook, NY) and maintained under anesthesia with 2% isoflurane in 100% O2.
Only the connections that are utilized are maintained, and this inevitable process is responsible for the dramatic windows for learning (e.g., second languages and musical instruments) that close soon after adolescence [ 46].
Understanding how this variation is maintained, and how it is utilized (or tolerated) by biological systems, depends on linking it to variation in phenotype.
The transgenic strain utilized (M77) was maintained through crosses with wild salmon and thus contain on average the same genetic background as the wild-type fish except for the presence of the OnMTGH1 transgene.
The LSU colony of cat fleas is maintained on shorthair cats which utilize shredded corn cob shavings in the litter box; the same shavings are the origin of the L. bostrychophila used for isolation of R. felis in the current study.
