Sentence examples for were maintained overnight from inspiring English sources

These conditions were maintained overnight to allow the fish to acclimate.

All cultures were maintained overnight then Alamar Blue (Biosource International) was added to a final concentration of 10% (v/v).

Cells were maintained overnight and the next day, switched to a differentiation medium consisting of growth medium with reduced FBS (2% final) and 8 µM retinoic acid (Sigma, St Louis, MO).

The plates were maintained overnight at room temperature on a shaker to dissolve the dye.

Prior to bloodfeeding, the mosquitoes (4 6 days post-eclosion) were maintained overnight on water only.

In fact, once testing was complete, the CF3 indomethacin-treated animals were maintained overnight for further observation.

IPTG was added to 0.5 mM and protein expression was maintained overnight at 20°C.

Insulin delivery was gradually reestablished by closed-loop therapy 1 h after the hypoglycemic episode was treated, allowing euglycemia to be maintained overnight.

The male rat at 48 h after treatment with siRNA was mated with normal female rats; they were maintained together overnight, vaginal smears of the females were taken in the following morning and examined microscopy; the mated male rat was sampled for determining the knockdown efficiency.

After electrospinning, samples were maintained under vacuum overnight to ensure complete solvent removal, photocrosslinked as above with ultraviolet light (Blak-Ray) in a nitrogen atmosphere, and incubated in PBS overnight.

These resuspended cells were maintained at -20°C overnight.