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The temperature and aeration rate were maintain at 28 °C and 3 L/min during the whole fermentation process, respectively.
For long term maintenance, cultures were maintain in 'basal' foregut media without NOG and SB, or in the presence of growth factors including 50, 500 ng/ml FGF2 (R&D systems), 10 µM Sant-2 (Stemgent), 10 µM SU5402 (SU, Stemgent), 100 ng/ml SHH (R&D systems), and SAG (Enzo Life Sciences, Farmingdale, NY) for 8 days.
Similar(57)
Buildings were maintained more regularly.
The effects were maintained at follow-up.
Civil liberties were maintained.
Thus, no official standings were maintained.
Mice were maintained under sterile conditions.
Age-matched rats were maintained as controls.
All incubation times were maintained constant.
Embryos were maintained as standard method.
All mice were maintained under SPF conditions.
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