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Mitochondria were labelled with an antibody against TOM20.
After annealing, oligonucleotide probes were labelled with P32-γ-ATP using T4 polynucleotide kinase.
Exosomes were labelled with the lipid dye PKH67 and incubated with MODE-K cells in vitro.
Vessels were labelled with Texas red and imaged with a two-photon microscope.
Both sense and antisense riboprobes were labelled with digoxigenin according to the manufacturer's instructions (Roche Farma).
AHA-incorporated proteins were labelled with biotin using Click-iT BInvitrogeneInvitrogens DetectInvitrogennvInvitrogen
To this end, mitochondria were labelled with the TOM20 antibody (Fig. 5Aa,b).
The released N-glycans were labelled with 2-aminobenzamide (2-AB) (Sigma-Aldrich).
Receptors were labelled with a 1 1 mixture of Alexa 488-Affibody and CF640R-Affibody.
Briefly, cells were labelled with BrdU (50 μM, Sigma, #B5002) for 2 h.
The cells were labelled with CTV and used as responder cells (Tresp).
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