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During the entire 2 day-cell culture on a chip, it was confirmed that the assembled hydrogel unit and the attached cells were kept stably under the flow of cell culture media (Fig. 5c).
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The activity could be kept stably after a long time storage or high temperature treatment.
Stably transfected clones were kept in culture for no longer than 20 passages.
Stably transfected hDAT CHO cells were kept under selection with 250 μg/mL hygromycin B Corning Cellgroo).
MCF-7 cells, the HCC1937 BRCA1-mutant breast cancer cell line (ATCC) and HCC1937 cells stably transfected with green fluorescent protein (GFP)- BRCA1 (gift from Dr. Ralph Scully) were kept in RPMI 1640 medium with 10% fetal bovine serum.
Initially, animals were kept in a 12-h light:12-h dark cycle (LD 12∶12 h) for several days until they were stably entrained.
Teeth were kept moist.
Data were kept confidential.
These were kept separate.
As shown in the figure, the robot walked stably at constant speed and the body posture was kept in almost horizontal.
Everything was kept apart.
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