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All sequences were inspected and validated at the genomic DNA level to remove wrongly predicted sequence regions, to manually fill gaps in gene predictions, and to reveal the correct exon/intron boundaries.
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To identify possible duplicated R. microplus miRNAs all reads mapped onto a single D. melanogaster miRNA locus were inspected and if miRNA isoforms were observed these were required to be cloned in at least two distinct libraries to be validated.
These alignments were inspected and manually refined.
Sequences were inspected and analyzed using Sequencher4.10™.
The animals were inspected and weighed daily.
Masks were inspected and manually corrected where necessary.
These alignments were inspected and modified where necessary.
Animals were inspected and weighed every three days.
Seeds were inspected and watered every 24 hrs.
Sites were inspected visually and validated functionally by measuring impedances in phosphorous-buffered saline solution (PBS).
The vaccination site was inspected and examined for remaining nodules.
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