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Catheters were inserted successfully in all cases.
Plasmids were purified from kanamycin-resistant colonies using the QIAprep Spin Miniprep Kit (Qiagen), and were sequenced to verify that the DNA sequences were inserted successfully into pDONR221.
All catheters were inserted successfully as day cases except four patients (13.3%) who had catheters inserted during an inpatient hospital admission.
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Buffer planning is also considered by deciding if buffers can be inserted successfully for each net.
However, PCR primers designed to amplify the sequence between 14271 bp and 14532 bp, 3' of the Sstr5 transcription initiation site (TS1F and TS1R), where the hCD3ε transgene is inserted, successfully amplified genomic DNA from tgε26+/+ as well as from WT mice (Fig. 2E, lanes 1 and 2).
Hoses were inserted.
Five hundred twenty-five out of 546 initially planned implants in 259 patients could be inserted into successfully augmented areas, whereas 21 implants in 20 patients due to graft loss could not be inserted.
In addition, C4-enzyme genes have been inserted into and successfully expressed in rice (Mitchell and Sheehy, 2006).
Foreign sequences have successfully been inserted into the nsp2 and N regions and in the space between ORF1b and ORF2a.
As Akatsuki has successfully been inserted in the Venus orbit in December 2015 (Nakamura et al. 2016), IR2 will remain a unique instrument that can obtain NIR 1.74- and 2.3-μm window images of Venus from the orbit in the coming years.
To this end, we selected the mouse Rosa26 locus, which exhibits apparently tissue-ubiquitous expression and into which reporter sequences have successfully been inserted downstream of the promoter (Zambrowicz et al., 1997; Soriano, 1999; Srinivas et al., 2001).
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CEO of Professional Science Editing for Scientists @ prosciediting.com