In the first set of proteasome inhibition experiments, the cells were inhibited for 0, 5, 10 and 20 hours, and cell lysis and protein purification were performed under non-denaturing conditions.
4-BPB is an irreversible inhibitor, and once parasites were pre-treated with it, they were inhibited for invasion for about 6 hrs, despite no drug being present.
Colony forming assay displayed a significant reduction in both the size and number of colonies when cells were inhibited for the expression of RECQ1 (Fig. 4C).
Although clotting activities of SCs from 19 strains of them were inhibited for at least 24 hr by pooled normal human gamma-globulin, with the 5 remaining strains, gamma-globulin also inhibited clotting but for a shorter period of time (4 5 hr).
For the measurement of maximal inhibitory concentrations (IC20, IC50, and IC80) blood samples were inhibited for 30 min at room temperature 25°C with appropriate concentration malathion compound, depending on preliminary range finding tests [ 2, 25].
Schmid et al. (1996) also observed that although the LPZ underwent reorganization in V1, the responses of the cells to the stimulation of the lesioned eye were inhibited for 10 s by the synaptic connections of the normal eye.
