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The mouse neuroblastoma cell line N2a (CCL-131, American type Culture Collection, Rockville, MD) was cultured and sibling clones were infected in parallel with brain homogenate from scrapie-infected mice (strain 22 L) or from healthy controls as previously described [ 15].
Mock-treated cells were infected in parallel.
For one-step growth kinetics analysis, confluent cell monolayers in 24-well plates were infected in parallel at an MOI of 3 CIU/cell.
HUVECs and keratinocytes were infected in parallel with retroviruses encoding KOSM and GFP on day 0 (1X), or day 0 and day 1 (2X), equivalent numbers of GFP positive cells plated, and resulting colonies stained for Nanog as an initial measure of pluripotency (Figure 2A, 2B).
HeLa cells were infected in parallel with the ΔsptP mutant or with the same mutant expressing the FLAG-tagged SptP variants listed in Figure S2B.
THP-1-derived macrophages were infected in parallel with Smooth and Rough variants of M. abscessus ATCC 19977-IP [ 11, 12].
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"He had been infected in Uganda.
How many have been infected in this way?
PHHs (donor 1 and 2) were infected and examined in parallel.
Control groups of C57BL/6 (B6) mice and B6129PF2 hybrid mice were also infected in parallel.
However, tube cultures are often infected in parallel to detect the low viral load specimens, which may not be detected by SV [ 6].
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