Thus, Pr160gag-pol was indeed incorporated into the virions and processed properly.
As shown in Fig 1B, the pellets from infected macrophages contained Anx2 as well as mature capsid (p24Gag), confirming that Anx2 was indeed incorporated into virions.
This finding raised two possibilities: Firstly, clathrin is associated with contaminants rather than the VLPs, or secondly, clathrin is indeed incorporated into VLPs but is exposed on the surface like HA and NA.
Kinetics over a 2 hr incubation period indicated that [α 32P] CMP was indeed incorporated into TCA precipitable material since cpm registered at all the time-points of the kinetics were over the background cpm registered at t = 0, reaching a mean 3 fold above background at 2 hr (Fig. 2A).
It was further found that the Pr160gag-pol was indeed incorporated into the virions of these two mutants, because except for RT, all other products of Pr160gag-pol including p24, protease and integrase [31], [32], could be detected in the viral particles of mutants Y271A and I271A (Fig. 4A).
To investigate whether the precursor Gag-Pol polyprotein was indeed incorporated into pseudoviral particles of mutants Y271A and I274A, products of the Gag-Pol polyprotein, integrase (IN), protease (PR) and p24, in wild type and mutant pseudoviral particles were examined by Western blotting.
The co-sedimentation of NefG3C-GFP and CAp24 strengthens the conclusion that NefG3C-GFP molecules are indeed incorporated in VLPs.
Is there any evidence that these CENP-A molecules are indeed incorporated into nucleosomes/chromatin (rather than being chromatin-associated in a less defined manner)?
Higher magnification of the interior of the film shows that it is composed of highly entangled and interwoven nanofibers that are clearly discernible indicating that RADA16-I is indeed incorporated into the film as nanofibers.
