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As controls, monocytes were incubated alone.
No haploid fruiting was observed when the samples were incubated alone on V8 juice agar.
As controls, monocytes, apoptotic or live Jurkat cells were incubated alone.
The pooled brain homogenates were incubated alone or with the antibody loaded beads at 4°C for 2 hrs.
In some experiments (Fig. 6), HIV-1 infected iDCs were incubated alone or with aNK cells for 3 days, in the presence of rh-HMGB1 (1 µg/ml) (R&DSystems), rabbit anti-HMGB1 Abs (10 µg/ml) (Abcam, Cambridge, UK) or Glycyrrhizin (10 µg/ml).
Overall, these data suggests that serial killing by NK cells is mediated by Perforin and Granzyme B. To investigate whether NK cells survive or undergo apoptosis after killing their targets, NK cells were incubated alone and with 221 targets for 16 hours.
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In this case, previously to the incubation with TTR, A-Beta peptide was incubated alone for aggregation purposes, and then further incubated in the presence or absence of TTR for another 3 hours.
Perlecan Dm1 purified from HEK293 cells was incubated alone (lanes 2, 9) or with heparitinases 1, 2, 3 and chondroitinase ABC either together (lanes 3, 10) or separately (lanes 5 8).
Monomeric Aβ42 (25 µM) was incubated alone or with different concentrations of fatty acids in a buffer containing 50 mM NaCl and 20 mM Tris pH 8.0.
At a 1.0 0.14 molar ratio of insulin: αB-crystallin the lag phase of aggregation was the same as when insulin was incubated alone (10 min), but the rate of aggregation was decreased such that the amount of protein precipitation after 90 min was reduced by 40±3% (Fig. 2C).
Whilst the I159A/I161A mutant delayed the onset of βL-crystallin's aggregation to 40 min, by the end of the assay the amount of light scattering in the presence of this mutant was similar to when βL-crystallin was incubated alone.
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