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Immunoprecipitates were immunoblotted as above.
TA muscle lysates were immunoblotted as described previously [6].
Membranes were immunoblotted as described earlier.
The samples were immunoblotted as previously described (Sandri et al., 2004) and visualized with SuperSignal West Pico Chemiluminescent substrate (Pierce).
Samples were immunoblotted as described above, using a monoclonal anti- α-syn antibody (1 : 1000; BD Biosciences).
Proteins were immunoblotted as described [ 29] by using anti-TG2 (ThermoScientific TG100; 1:500), anti-LAP (R&D Systems Ab-246-NA; 1 1,000) or anti-actin (Sigma Ab A5060; 1 5,000) antibodies.
Similar(52)
The membrane was then blocked again in 5% milk in TBST, and finally it was immunoblotted as described above with the appropriate primary and secondary antibodies.
The products were immunoblotted using anti-HA to evaluate K63-linked ubiquitinated proteins (upper panels).
The in vitro translated products were immunoblotted with anti-Cdx4 antibody.
The pulldown eluates were immunoblotted and Rac proteins detected using GFP specific monoclonal antibodies.
Immunoprecipitants were immunoblotted (IB) for cavin-3 and myosin-1.
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