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Once back on the ground, samples were immediately processed for microscopy.
Samples were immediately processed for physical and mycological analyses and kept at −4°C until FB1, AFB1, and OTA analyses.
After 2 h of reoxygenation liver tissue samples were immediately processed for histological and biochemical analyses of markers of oxidative stress and tissue injury.
For RNA extraction, samples were immediately processed after collection.
Tissues were immediately processed for mRNA, protein isolation and immunoprecipitation.
Plasma samples were immediately processed and stored at −70°C until the analyses were performed.
Human brain tumor biopsies were immediately processed for EGFR targeted QD-staining.
After collection, the samples were immediately processed and stored at −80°C until RNA extraction.
The sorted cells were immediately processed to purify the RNA, which was analyzed by quantitative real-time PCR.
In parallel, blood samples were immediately processed for the isolation of Peripheral Blood Mononuclear Cells (PBMCs) by density gradient centrifugation using Ficoll-Hypaque (Sigma).
The lysates were used to evaluate the expression levels of HIF1α by western blotting and frozen tumor sections were immediately processed for immunofluorescence analysis.
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