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2 3 pools, identical to those used in Affymetrix hybridizations, were hybridized together with a reference pool (pool of all samples) to Sterolgene arrays.
All microarrays were hybridized together.
In centers 2 and 3, female and male samples were processed separately at the washing step, and in centers 4 and 5, samples were hybridized together in groups defined by strain.
BACs were hybridized together with chromosome-specific painting probes.
Labeled samples were hybridized together to mouse 2.1-million probe promoter arrays (Roche Nimblegen, Madison WI).
In the following step, individual cDNA-samples were hybridized together with the differently labeled reference cDNA on the microarray.
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It has to be taken into account that carbon atoms are hybridized together in the form of sp, s p2, and s p3.
In a two-color microarray experiment, we consider the issues of determination of which mRNA samples are to be labeled with which fluorescent dye and which mRNA samples are to be hybridized together on the same slide.
The numbers of array that can be hybridized together is constrained along with several other steps in sample processing.
The reference cDNA was hybridized together with the Cy5-labeled cDNA synthesized from RNA extracted from cells grown under experimental multiple-stress conditions.
For multi-channel arrays multiple conditions are hybridized together on individual arrays using multiple dyes.
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