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Spleen samples (n = 4) were homogenized by glass dounce homogenizers in Hank's buffered saline to result in a single cell suspension.
Prostate tissues were homogenized by a tissue homogenizer in protein lysis buffer.
Briefly, BATs were homogenized by sonication in homogenization buffer (1 N HCl, 0.25 M EDTA, 1 mM Na2S2O5).
The organs were homogenized by using a glass homogenizer.
Tissues were homogenized by using a Polytron PT3000 homogenizer (Kinematica).
Tissue samples were homogenized by hand in ground glass homogenizers (Kontes, size 22) on ice in 1.0 to 2.0 ml saline (0.9% NaCl).
They were homogenized by a Fast Prep®-24 homogenizer (MP Bio).
After spheroplasting, cells were homogenized by 25 strokes in a Dounce homogenizer.
Samples of brain, muscle and liver were homogenized by measn of a bead mill homogenizer (45 sec at 3000 oscillations per sec, Mixer Mill MM 200, Retsch).
The cells were homogenized by douncing three times in a dounce homogenizer with a sandpaper-polished pestle.
After incubation on ice for 10 min, the cells were homogenized by 20 strokes in a tightly fitting Dounce homogenizer.
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