The outcome of this procedure produced many islet-like cell clusters, which could express insulin and glucagon as shown in figures 1 and 2. For in vitro insulin secretion assay, twenty stage 4 cell clusters were handpicked, washed with PBS 5 times to prevent insulin assay cross reaction with medium, and then cultured in 2% FBS/DMEM (5.5 mM glucose) without B27 and nicotinamide for 24 h.
The first recipients were handpicked after discussions with local organizations.
The Republicans who met with Mr. Bush were handpicked and the meeting was largely set up by Charles Francis, a gay public relations consultant who is the brother of James B. Francis, Jr. the chairman of the Texas Department of Public Safety.
After several washes with HBSS, islets were handpicked under a microscope.
Pancreatic islets were isolated by collagenase digestion and the islets were handpicked in cold Hank's buffer with 1 mg/mL bovine serum albumin (BSA) prior to secretion assays.
Pancreatic islets were handpicked under a stereo microscope, counted and subsequently washed with HBSS buffer (Invitrogen, CA, USA) as previously described [ 87].
Auditors, however, determined that the system might not have been random at all, with investigators being told that candidates instead were handpicked by managers.
With this in mind, all staff within CBS were handpicked from our local community and all services and supplies sourced locally wherever possible.
Islets were handpicked under a microscope, counted and cultured in RPMI 1640 (MP Biomedicals, Santa Ana, CA) supplied with 10% FBS (ThermoFisher) in Petri dishes at 37 °C and 5% CO2.
Individual pancreatic islets were handpicked and cultured in Dulbecco's modified eagle medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 100 U/mL streptomycin (GIBCO, Grand Island, NY), 5 μg/mL penicillin, and 10% (v/v) FCS at 37°C.
