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To generate functional rTFs, the 3F peptides were fused to either VP16 or VP64 AD.
Split TEVp fragments were fused to either FK506 binding protein (FKBP12) or the FKBP12-rapamycin-binding domain of FRAP (FRB).
Peptides of angiotensin II, S.typhi outer membrane protein (D2), CXCR4 receptor, HIV1 Nef, gonadotropin releasing hormone (GnRH), Influenza A M2-protein were fused to either N- or C-terminus of AP205 coat protein.
These were fused to either GFP or RFP as described for ATM1.
The optimised nuclear localisation signal (NLS) from the SV40 large tumour antigen (Op-T-NLS) or the C-terminus of Apoptin (residues 74 121; tNTS) were fused to either histone H2B or histone H3 using primers designed for overlap extension polymerase chain reactions.
The fluorescent proteins were fused to either the N- or the C-terminus of AtBPC6 and analyzed for GFP- or RFP-emission under GFP-excitation light.
Similar(54)
mCherry [43] was fused to either N-terminus (α-tubulin) or C terminus (EB1 and LIC1).
Identical DmsAL peptide constructs have been fused to either GST or SBP.
For both formats and TCRs, TCRα was fused to either Ig heavy or light chain, as was TCRβ.
TMPRSS2 was fused to either ERG or ETV1, two members of the ETS family of oncogenes (Tomlins et al, 2005).
This system is convenient for genetic manipulation because CBM has a small molecular weight and target proteins can be fused to either its N or C terminus.
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