Strains that were susceptible to optochin but for which no serotype was assigned were further tested by using the AccuProbe Streptococcus pneumoniae Culture Identification Test (Gen-Probe, San Diego, CA, USA) to confirm species identification.
The generated models were further tested by use of an external prediction set of 15 molecules.
They were further tested by FTIR, DSC, CD, tensile strength measurement, water uptake and swelling behavior.
At last, the proposed model and solution were further tested by a larger scale case.
Resistant plants were further tested by tissue PCR31 to verify correct integration of the construct into the genome, by amplifying the junction regions between the insert and the sequence flanking the deleted fragment at both the 5′ and 3′ ends (primers listed in Supplementary Table 2).
The significant differences, if any, were further tested by using Post Hoc test to reveal the effects of the method used, the epoch number, or the measuring time, which is responsible for the significant differences.
Secondary screening: After primary screening test, seven microbes with higher potency indexes and D p values were further tested by submerged fermentation at 45 °C.
QTL regions validated to participate in drought response in the co-localization procedure were further tested by adding markers contiguous to their locations.
Positive samples were further tested by RT-PCR amplification and sequencing, as well as electron microscopy.
Phage clones were further tested by ELISA for their ability to bind specifically to C. albicans.
Selected cytokines were further tested by standard ELISA on pooled tear samples.
