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Both models were further subject to extensive Molecular Dynamics (MD) simulation studies (GROMACS 4.5.3 was used).
After the completion of chemical synthesis, all the compounds were evaluated through in vitro antibacterial activity assay, some of which were further subject to in vivo rat pharmacokinetic assessment.
These data were further subject to an evolutionary analysis ranging from yeast to human, leading to the identification of putative clusters of orthologous groups (COGs) of this gene family.
SNPs overlapping with an EST were further subject to allele frequency analysis using dbEST tissue information.
The RNA samples were further subject to DNase I digestion using a DNA-free kit (Ambion).
To obtain pure circular plasmid DNA, the vectors were further subject to a CsCl-ethidium bromide density gradient centrifugation.
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A total of 20 fractions were further subjected to nanoLC MS/MS analysis.
In case of positive cultures, the bacteria were further subjected to identification and antibiotic susceptibility testing.
Only the stable dispersions were further subjected to rheological examinations using the AWS rotational viscometry.
Isolates obtained were further subjected for the quantitative detection of laccase under solid-state fermentation.
Slabs containing 2% of fibers were further subjected to a non-deformable projectile impact.
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