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Biotinylation nanobodies were further purified on a Streptavidin Mutein Matrix.
The induced nanobodies were further purified by Ni2+-IDA column.
Three fractions were further purified by Sephadex G-100.
Thus, the two fractions were further purified by different separation methods.
The samples were further purified by drying in vacuo at 60 °C for approximately 4 h.
The resultant solid products were further purified by dialysis and ultrafiltration for cell imaging.
The proteins were further purified separately by 1 mL Hitrap Q HP column (GE Healthcare).
Morphologically distinct individual colonies were further purified on LB agar plates.
The peptides were further purified from protein hydrolysates through using an ultrafiltration membrane with MWCO of 2 kDa.
The identified proteins were further purified through gel extraction and recovery of protein varied from 80 to 95%.
The proteins were further purified by Ni-NTA affinity chromatography and size exclusion chromatography (See "MATERIALS AND METHOD S).
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