Numbered bands were further identified by sequencing (Table 3).
The pigments were further identified by means of LC MS (Additional file 4: Figure S3).
Anthracene and naphthalene were further identified by comparing UV spectra and retention times with the standards.
These pigments were further identified by LC MS, which determined the molecular weights of Y1, Y2, Y3 and Y4 as 250, 254, 402 and 358, respectively (Table 2).
The haploid plants were further identified by botanical traits, cytology, cytogenetics, and molecular marker, and will be potentially used for the cloning of MS2 dominant male gene.
The interacting nucleotides in such an interaction were further identified by generating footprints of the complex by Pb II -induced hydrolysis.
Moreover, the selected two non-sporulating fungi (SaR-3, SaR-6), Alternaria sp. (SaF-2), and Fusarium sp. (SaR-2) were further identified by the internal transcribed spacer (ITS) rRNA gene sequence analysis.
23 of 25 tested colonies displayed the desired phenotype; 5 of these were further identified by PCR, with loss of the plasmid p148-cre because of the plasmid instability.
Using the gene-deletion plasmid and ATMT, transformants were selected on PDA plates with 100 µg/mL hygromycin B. Then, these transformants were further identified by PCR using the primers P11N-R/P11N-F P11N-R/P11N-F P11N-R/P11N-F-F (Additional file 3: Table S3) to verify Δtri11, Δtri3, and Δtri4 mutants, respectively.
After screening yeast strains with Sudan III, morphological and physiological characterization and identification of such potential oleaginous yeasts, they were further identified by sequence analysis of the ITS and the D1/D2 domains of the 26S rRNA gene and the sequences obtained from this study and that of closely related sequences from GenBank are displayed in Fig. 2.
Bands corresponding to NS5BΔ21for all five genotypes were further identified by Western blot analyses with a polyclonal antibody directed against the NS5B from genotype 1b (Fig. 3B).
