Following requests by Singaporeans, guidelines for the use of the flag were further broadened in 2006 to give residents a variety of opportunities to express their loyalty to Singapore during National Day celebrations such as the National Day Parade.
Those receiving ET were further identified by individual flags for ECMO and RRT, respectively.
In addition, Flag-MTA1 and its deletion mutants were further purified from whole cell extracts using anti-Flag M2 agarose beads as described [ 54].
The whole cell lysates were further subjected to immunoprecipitation with anti-Flag M2 agarose and analyzed by Western blotting using an anti-HA or an anti-FLAG antibody.
We searched the possible rice orthologs for the Arabidopsis starch-related genes, and made a rice gene-expression profile for the diurnal changes of sucrose metabolism-related enzymes in seedling and flag leaves, which were further displayed by MapMan (Version 3.0).
After filtering the data initially for flags, 10,765 probes were further filtered for ANOVA.
These interactions were further confirmed by immunoprecipitation of HA-Wss1 from DOA1-FLAG cell lysates.
This list was further filtrated using the flags from the MAS5 normalization.
As expected, the piRNA-CAF1 association could be further enhanced by overexpressed Flag-MIWI.
Jobs that are disliked/reported are flagged to the Pickle team, who investigate the issue further.
