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The prepared virus stock dilutions were further diluted in CMV-negative human ethylenediaminetetraacetic acid plasma.
To reach the desired sperm concentrations and oocyte:sperm ratios, the sperm dilutions were further diluted in IVF medium.
Weak internal structures were further diluted with aggressive external pressures.
All solutions were further diluted in saline.
These solutions were further diluted by addition of saline solution (0.9 % NaCl w/v, 1.1 mL).
Before the experiment drugs were further diluted with SIF to their final concentration.
These solutions were further diluted to get (0 100 µM) concentration.
The suspension were further diluted 1 100 to obtain density of 105-106 cells/mL.
The stock solutions were further diluted with the mobile phase to obtain the working concentration ranges.
The stock solutions were further diluted with deionized water to the desired concentration for obtaining the test solutions.
The resulting mixtures were further diluted with distilled water and the absorbance was recorded spectrophotometrically at 225 nm.
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