Sentence examples for were further concentrated from inspiring English sources

Purified Fc fusion proteins NFc-CXCLC in Buffer T were further concentrated to 2 mg mL−1 by using 10000 NMWL Amicon Ultra-15 ultrafiltration devices at 4000 × g and 4 °C on a Allegra X-14R centrifuge and cleaved by using recombinant TEV protease (0.5 mg mL−1).

The purified NCXCL-G2-AviTagC proteins were further concentrated by using a 3000 NMWL Amicon Ultra-15 ultrafiltration devices (Millipore) at 4000 × g and 4 °C on a Allegra X-14R centrifuge and subjected to SEC by using a HiLoad 16/600 Superdex 75 prep-grade column (GE Healthcare) equilibrated with biotinylation Buffer R (50 mM Bicine, pH 8.3) on an AKTApurifier system.

The final purified and biotinylated proteins were further concentrated by using 3000 NMWL Amicon Ultra-0.5 centrifugal filter units (Millipore) at 14,000 × g and 4 °C on a Eppendorf 5702 R centrifuge (Eppendorf) to a final protein concentration of approximately 100 μM, flash frozen in liquid nitrogen, and stored at −80 °C.

The ethyl acetate extracts were further concentrated using a rotary evaporator.

The modified products were further concentrated under vacuum to 50 mL by using a rotary evaporator (EYELA, Tokyo, Japan) at 47 °C.

Both these fractions were further concentrated by lyophilization; these samples of identical protein amounts (50 µg) were denatured and run through sodium dodecyl sulfate polyacrylamide gel electrophoresis (12%%).

By contrast, Mn in Rubus foliage was up to 100 times more concentrated than in most plant species, and was further concentrated by ∼60% during senescence.

If the final protein concentration was below 1 mg/ml, the preparation was further concentrated using Vivaspin-6 or -20 (5000 MW PES, Sartorius AG, Göttingen, Germany).

The gold was concentrated by washing away the lighter river sands with water, leaving behind the dense gold particles, which could then be further concentrated by melting.

A change in corporate structure had stripped minority shareholders of much power, which was further concentrated in the hands of the Farahs, according to one internal document.

Marcolino Moco, who served as a prime minister and secretary general of the ruling party under Mr. dos Santos but is now a government critic, said that during the oil and reconstruction boom in recent years, power was further concentrated in the president's hands — fueling corrupt practices in his inner circle.