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Then, 1H NMR spectral data of compound 2 were further collected in different solvent, and the result indicated same coupling pattern without large coupling constant to meet Ortho-proton in aromatic ring.
Additional information concerning the viruses, such as genome organization, average size and virion illustrations, and the bats, including common names, diet type and geographic distribution, were further collected from the ViralZone database (25) and the ICUN Red List (www.iucnredlist.org), respectively.
The cells were further collected and lysed in Nu-SDS PAGE buffer followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis using Rb anti-FLAG antibody (1 1000; Sigma-Aldrich).
Blood samples were further collected for glucose and insulin at 30, 60, 90, and 120 min and C-peptide at 120 min. All subjects were discharged after 10 14 days of intensive insulin therapy and then randomized into two groups: continuing with insulin treatment or shifting to OADs.
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A plot of the specific capacitance versus current density (Fig. 5b) was further collected to study the rate performance.
The dust was captured by creating suitable air stream at milling station which was further collected in a dust collector with HEPA (High-efficiency particulate arrestance) filter.
Moreover, other than facilitating accurate timing, this highly strong single-frequency component can be easily extracted from received signals by using a simple PLL, so that the recovered energy can be further collected to compensate energy consumption in receivers [18].
Epidemiological background of these patients was further collected.
All taxa flagged as high priority in steps (2) and (3) are included in the list of high priority taxa to be further collected.
Information about the density of physician and nurses each country was further collected from the WHO website.
The supernatant was further collected by ultracentrifugation in a Beckman SW 40Ti rotor at an average of 150, 000 × g for 1 h at 4°C.
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