These DNAs are mutant for λ recombination functions, including orf and rap, which were functional in previous studies.
W65R and V69M mutations were found in GLUT1 variants, which were functional in yeast32.
They were functional in design and ascetic in appearance, incorporating rigorous geometric forms and bare facades.
Among L. plantarum PEP-PTS genes, SacALP and SacKLP were functional in E. coli K12.
Promoters, selectable markers and plasmid replication proteins from C. bescii were functional in C. thermocellum.
Subsequent investigation found that of 67 pumps on the East Bank of the city, just 58 were "functional in some form".
Having demonstrated that crHybs were functional in vitro, we questioned whether the same would be true for the tracrRNA molecule, again with the potential for reduced synthesis costs and new properties.
The pCsgD*EFG plasmid complemented ∆csgE, ∆csgF, and ∆csgG (Supplementary Fig. 7), indicating that pCsgDEFG and pCsgD*EFG were functional in the respective mutant strains and that active CsgD, CsgE, CsgF, and CsgG were required in the ∆csgD strain for curli production.
It was found that several known IRES-containing mRNAs, such as BiP and c-myc, were both associated with the translation apparatus and translated in infected cells when cap-dependent translation of most host-cell mRNAs was blocked, indicating that the IRES elements were functional in their natural mRNAs.
A majority of the promoters were functional in vivo and seven were identified to drive expression of the fluorescent reporter better than the current best endogenous promoter in C. reinhardtii, the chimeric hsp70/rbs2 promoter.
Only those G beta gamma complexes containing a prenylated G gamma subunit were functional in this assay.
