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All samples were frozen upon arrival to reduce the effect of potential degradation due to storage.
RT4-D6P2T cells were cultured for less than 2 months after reconstitution from stocks, which were frozen upon receipt from the ATCC.
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Suppressing my fear of being frozen upon entry, I opened the door and beheld a storeroom filled with boxes of expired vitamins from the "nutritional supplement" store upstairs.
He drinks healthy smoothies and takes vitamin injections, but he's been smoking for over forty years (perhaps he figures that, if he's frozen upon death like he plans, he can get his lungs cleaned up in the future).
Samples were shipped by courier to the Paterson Institute for Cancer Research in Manchester in solid carbon dioxide, and confirmed to be frozen upon arrival.
Because the inocula had been frozen upon collection, 100-mL aliquots of each were defrosted at 30°C and then immediately placed under anaerobic conditions into sterile pre-reduced 500 mL Duran bottles.
All swabs were frozen (−20°C) upon collection from healthy individuals.
Cell lysates were frozen immediately upon collection.
Sera were frozen (−20°C) upon collection and stored in a bio-bank at the Geneva University Hospitals.
Cell lysates were frozen immediately upon collection; following thawing, they were clarified by centrifugation at 10,000 g for 5 minutes at 4 °C prior to use.
Samples from A and B were frozen at −80°C upon collection, whereas samples C and D were processed fresh from the same day as collection.
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