Sentence examples for were frozen down from inspiring English sources

On the same time we strongly suggest the further use of the same cell lines been transfected in this studies in Orthotopic Xenotransplantation Studies, as we best stable cells were frozen down already.

Aliquots were frozen down and thawed as needed for infections.

Cells were frozen down in multiple aliquots at passage 3 to 7, and stored in liquid nitrogen until use.

When cultures reached confulence cells were frozen down in liquid nitrogen until further use.

Initially, cells were frozen down after every passage, however after passage 20 this was reduced to every five passages.

Cells were washed in water and pellets of 1 2 × 10 cells were frozen down in liquid nitrogen and stored at 80°C until analysed.

One of the resulting plates was frozen down and screening of clones was performed by Southern blots on the other plate, following digestion of isolated genomic DNA with BamHI or EcoRV and using "Rosa26-5'", a 502 bp probe, located upstream of the insert and amplified from the Rosa26 locus using the primers 5'-GATAGGAACTGGAAAACCAGAGGA-3' and 5'-GTAAGGGTCCAACAGAAAAGAGA-3' (Fig. 1A).

A sample was frozen down at each serial dilution.

Every time a batch of cells is frozen down, it is recommended that one vial is resuscitated immediately to check viability.

After one week, the fibroblasts grew out from the NPC biopsies will be frozen down in liquid nitrogen for future use.

After 30 min of rotation in the cold room and subsequent centrifugation, protein was quantified and 20 µg of protein was frozen down as input controls.