Sentence examples for were freshly added from inspiring English sources

The serosal media were freshly added and collected daily for IL-8 determination.

When solutions were freshly added by the progression of evolutionary searches, the value of VarS increased, showing that some new solutions are distant from the centroid of the employed clusters.

NAC andNAA (Sigma-Aldrich, St . Louis MO, USA) were freshly added every 48 h to the culture medium at 5 mM final concentration.

To access the effect of later inhibition of JNK during TGF-β-induced EMT, NMuMG cells were treated starting at day 1 or day 3 after TGF-β stimulation; medium, inhibitor, and TGF-β were freshly added every second day.

Nox4 inhibitors, VCC300991 : 01 and VCC444973 : 02, were synthesized by Vichem Chemie Ltd ,Budapest, Hungary and were freshly added every 48 h at 5  μM final concentrations (unless otherwise indicated).

Final concentrations of 1 pg/ml to 10 ng/ml PGE2 (Sigma Chemical Co., St .Louis, Mo, USA) or 5 ng/ml TGF-β2 (R&D Systems, Minneapolis, MN, USA) were freshly added to medium A from day 0 at each medium change.

AZT Pfizerr) was freshly added (1∶1000 dilution from frozen stocks kept in −80°C) daily and continuously applied to the serosal compartment, in the absence or presence of mucosal exposure to 30 µl PBS or SMM, throughout the course of the studies.

Cells were washed twice with cold PBS and subsequently incubated on ice for 10 min with cold nuclei lysis buffer (50 mM Tris HCl, 10 mM EDTA, 1% SDS) to which 1 mM protease inhibitors (Sigmafast, Sigma Aldrich, St Louis, MO) and 4 mM PMSF (Sigma Aldrich, St Louis, MO) was freshly added.

5 mM NADPH was freshly added to the pipette solution before experiments.

0.1 mM DTT was freshly added to the bath solution before experiments.

Nile red was freshly added to the staining solution from a concentrated stock (0.1 g mL-1 in acetone).