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Several labelled mitochondria appeared swollen (Fig. 5E) and were frequently aggregated in clusters or apposed at the inner membrane of vacuoles (Fig. 5D, G).
and then disseminated to the liver, lungs and kidneys of almost all animals within 96 h p.i.. Leptospires were also detected engulfed in the swelling vascular endothelial cells and were frequently aggregated around the capillaries in the dermis and subcutaneous tissue under the inoculated site.
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The bacilli predominantly existed as single organisms however various sized aggregates were frequently seen.
Finally, woodlice leaving a secondary aggregate were frequently observed walking in the arena in the high brightness whereas in low and medium brightness these woodlice had generally joined the final aggregate by the end of the experiments.
In addition, perivascular lymph aggregates were frequently found in the lungs of Pla2g3-Tg mice compared with WT mice.
Notably, these remaining aggregates were frequently surrounded by double membrane (Fig 6G and H), that indicates their transformation into an autophagic vacuole.
Some representatives of the Bacteroidetes phylum such as Flavobacteriaceae were frequently found attached to aggregates and appeared during an algae-bloom collapse [ 5].
Epidermal aggregates and edema were frequently noted, often at the tip of the tail and in the ICM.
In the other pigs in this group, which survived for 30 or 60 days, in the atrophic liver lobes, large aggregates of microspheres were frequently seen, associated with large areas of coagulative necrosis (often with yellow discoloration by bile imbibition) and surrounded by multinucleated giant cells, pigmented macrophages, and a fibrous capsule (Fig. 4e).
ELSs were frequently present within granulomatous inflammation, ranging from aggregates to highly organized forms.
Moreover, as previously reported [ 33], numerous autophagic vacuoles and parallel aggregates of intermediate filaments were frequently observed in expanded endothelial cells (data not shown).
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